Anolis sagrei stage 4 - 19 Heads

 

Anolis sagrei stage 5 - Head - 3D model by SangerLab (@SangerLab) [a2b656b]

Stage 5

Upper Jaw: Fronto-nasal prominence (the area of where the face will form anterior to the telencephalon) is prominent. Maxillary processes are small not extending beyond the anterior eye.

Lower Jaw: Mandibular process extends rostrally.

Eye: Differentiation of Lens.

 

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei head - stage 6 - 3D model by SangerLab (@SangerLab) [e2a9fd3]

Stage 6

Upper Jaw: Maxillary process extends rostrally, extending beneath the telencephalon. Fronto- nasal process is now present, appearing bifurcated into two large projections.

Lower Jaw: Mandibular processes roughly at level with center of eye.

Eye: Continued differentiation of lens. No eyelid visible.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei head - stage 7 - 3D model by SangerLab (@SangerLab) [ba44f3d]

Stage 7

Upper Jaw: Maxillary processes nearly reach the underside of fronto-nasal process. The bifurcation of the fronto-nasal processes appears reduced.

Lower Jaw: Extension of mandibular process to anterior margin of eye.

Eye: Optic cup ovoid in shape.

Anolis sagrei stage 8 - Head - 3D model by SangerLab (@SangerLab) [ed03388]

Stage 8

Upper Jaw: Maxillary process is in contact and may begin to fuse with fronto-nasal process.

Lower Jaw: Mandibular process extends rostrally to anterior edge of eye.

Eye: Optic cup still ovoid in shape. The earliest signs that the ectoderm is growing over the eye to become an eyelid is apparent.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei head - stage 9 - 3D model by SangerLab (@SangerLab) [454d98a]

Stage 9

Upper Jaw: Fronto-nasal process and maxillary processes now fused creating first evidence of a forward-facing snout anterior to eye.

Lower Jaw: Mandible still not level with the snout.

Eye: Upper and lower eyelids now distinct covering 10% of the eye.

New! Anolis sagrei stage 10 - face - 3D model by SangerLab (@SangerLab) [0495bd7]

Stage 10

Upper Jaw: Upper jaw is of equal length to lower jaw.

Lower Jaw: Lower jaw is of equal length to upper jaw.

Eye: Eyelids cover 25% of the eye.

Anolis sagrei stage 11 - head - 3D model by SangerLab (@SangerLab) [9d738f6]

Stage 11

Eye: Eyelids cover 75% of the eye.

Anolis sagrei stage 12 - head - 3D model by SangerLab (@SangerLab) [a00fca5]

Stage 12

Eye: Half of eye covered by eyelids.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei head - stage 13 - 3D model by SangerLab (@SangerLab) [3ab701f]

Stage 13

This stage is defined based on characteristics of scale and pigmentation development, which are not visible in the CT scans.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei head - stage 14 - 3D model by SangerLab (@SangerLab) [0f56f14]

Stage 14

This stage is defined based on characteristics of scale and pigmentation development, which are not visible in the CT scans.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size - Anolis sagrei stage 15 - head - 3D model by SangerLab (@SangerLab) [7f35dc1]

Stage 15

This stage is defined based on characteristics of scale and pigmentation development, which are not visible in the CT scans.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei head - stage 16 - 3D model by SangerLab (@SangerLab) [e65915e]

Stage 16

This stage is defined based on characteristics of scale and pigmentation development, which are not visible in the CT scans.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei head - stage 17 - 3D model by SangerLab (@SangerLab) [d1f575e]

Stage 17

This stage is defined based on characteristics of scale and pigmentation development, which are not visible in the CT scans.

Anolis sagrei stage 18 - head - 3D model by SangerLab (@SangerLab) [d74d965]

Stage 18

This stage is defined based on characteristics of scale and pigmentation development, which are not visible in the CT scans.

 

Anolis sagrei stage 19 head - 3D model by SangerLab (@SangerLab) [ec2a823]

Stage 19

This stage is defined based on characteristics of scale and pigmentation development, which are not visible in the CT scans.